5YZJ

Crystal structure of human Archease mutant D51A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	48.465, 59.587, 132.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.180 - 2.790
Rwork	0.177
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yz1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.790	2.790
Number of reflections	10143
<I/σ(I)>	34.748
Completeness [%]	99.9
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	30% PEG 400, 100mM HEPES/Sodium hydroxide pH 7.5, 200mM Magnesium chloride