5YZI

Crystal Structure of Mouse Cytosolic Isocitrate Dehydrogenase complexed with Cadmium

Replaces:  2CMV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	98.246, 91.428, 109.724
Unit cell angles	90.00, 113.65, 90.00

Refinement procedure

Resolution	28.866 - 2.519
R-factor	0.1905
Rwork	0.188
R-free	0.23130
RMSD bond length	0.004
RMSD bond angle	0.749
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	29260
<I/σ(I)>	22.7
Completeness [%]	97.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	0.1M sodium citrate pH 5.5 ,16% PEG 4000 , 20% isopropanol