5YZ5

Crystal Structure of Human CRMP-2 with T509D-T514D-S518D-S522D mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-12
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	114.117, 114.117, 196.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.224 - 1.800
R-factor	0.1874
Rwork	0.186
R-free	0.21900
RMSD bond length	0.007
RMSD bond angle	0.829
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.055	1.116
Rmeas	0.059	1.199
Number of reflections	114502
<I/σ(I)>	19.8	1.89
Completeness [%]	99.8	99.8
Redundancy	7.6	7.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	12% PEG 4000, 0.1M sodium citrate pH 5.6, 0.1M lithium sulfate