5YZ4
Structure of the PIN domain endonuclease Utp24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979135 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.740, 73.328, 74.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.591 - 2.135 |
R-factor | 0.2282 |
Rwork | 0.222 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mj7 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.903 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.170 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.184 | 1.000 |
Number of reflections | 7270 | 284 |
<I/σ(I)> | 18.9 | 1.9 |
Completeness [%] | 94.5 | 73.6 |
Redundancy | 12.1 | 7.3 |
CC(1/2) | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M calcium acetate and 20% polyethylene glycol 3350 |