5YZ2
the cystathionine-beta-synthase (CBS) domain of magnesium and cobalt efflux protein CorC in complex with both C2'- and C3'-endo AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.898, 56.290, 54.349 |
| Unit cell angles | 90.00, 114.73, 90.00 |
Refinement procedure
| Resolution | 48.954 - 1.750 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hg0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.759 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.766 |
| Number of reflections | 29796 | 2139 |
| <I/σ(I)> | 21.04 | |
| Completeness [%] | 99.3 | 97.2 |
| Redundancy | 6.5 | 6 |
| CC(1/2) | 0.999 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Bis-Tris pH5.5, 25% PEG 3350, 0.2M Nacl |
