5YYX
Crystal Structure of the MEK1 FHA domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97736 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.666, 61.666, 61.078 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.204 - 1.684 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g6g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.906 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.680 | 4.140 | 1.680 |
Rmerge | 0.082 | 0.051 | 0.589 |
Rmeas | 0.084 | 0.053 | 0.606 |
Rpim | 0.019 | 0.012 | 0.140 |
Total number of observations | 303239 | ||
Number of reflections | 15632 | 1134 | 936 |
<I/σ(I)> | 4.7 | ||
Completeness [%] | 99.4 | 100 | 91.1 |
Redundancy | 19.4 | 17.9 | 18.3 |
CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% PEG8000, 0.1M Imidazole malate pH7.5, 1.0M Lithium sulfate |