5YYM
Crystal structures of E.coli arginyl-trna synthetase (argrs) in complex with substrate Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.727, 244.510, 52.460 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 40.750 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bs2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.306 | |
| Number of reflections | 57753 | |
| <I/σ(I)> | 7.5 | 2.9 |
| Completeness [%] | 91.5 | 88.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% PEG8 000, 0.1 M NaH2PO4/Na2HPO4 pH 6.5 |
