5YY6

Crystal structure of Arabidopsis thaliana HPPD truncated mutant complexed with Benquitrione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97775
Spacegroup name	C 1 2 1
Unit cell lengths	77.333, 83.883, 66.310
Unit cell angles	90.00, 100.17, 90.00

Refinement procedure

Resolution	45.722 - 2.401
R-factor	0.1924
Rwork	0.190
R-free	0.24380
RMSD bond length	0.005
RMSD bond angle	0.734
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.487
High resolution limit [Å]	2.401	2.401
Number of reflections	15659
<I/σ(I)>	19.76
Completeness [%]	96.0
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.16	PEG 400, sodium chloride, Sodium acetate