5YY5
Structural definition of a unique neutralization epitope on the receptor-binding domain of MERS-CoV spike glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | H 3 |
| Unit cell lengths | 152.315, 152.315, 148.535 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.510 - 2.800 |
| R-factor | 0.2476 |
| Rwork | 0.246 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 31541 | 31541 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291.15 | tris pH7.5, polyethylene glycol 8000, ethylene glycol |
