5YY0
Crystal structure of the HyhL-HypA complex (form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-12-08 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 3 |
Unit cell lengths | 132.046, 132.046, 132.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.015 - 3.243 |
R-factor | 0.2918 |
Rwork | 0.290 |
R-free | 0.31370 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.793 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.015 | 3.360 |
High resolution limit [Å] | 3.243 | 3.243 |
Rmerge | 0.113 | 1.622 |
Number of reflections | 12459 | |
<I/σ(I)> | 23.6 | |
Completeness [%] | 100.0 | |
Redundancy | 20.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1M NaCl, 0.1M MgCl2, 12% PEG 3350, 0.1M imidazole-HCl (pH 6.2) |