5YXU
an affinity enhanced T cell receptor in complex with HLA-A0201 restricted HCV NS3 peptide KLVALGINAV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-03 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.106, 223.107, 71.855 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 17.700 - 2.700 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28870 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 48316 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG 3350, 0.2M potassium nitrate |
