5YXU

an affinity enhanced T cell receptor in complex with HLA-A0201 restricted HCV NS3 peptide KLVALGINAV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-03
Detector	OXFORD RUBY CCD
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	60.106, 223.107, 71.855
Unit cell angles	90.00, 98.30, 90.00

Refinement procedure

Resolution	17.700 - 2.700
R-factor	0.238
Rwork	0.235
R-free	0.28870
RMSD bond length	0.010
RMSD bond angle	1.449
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	48316
<I/σ(I)>	6.6
Completeness [%]	99.5
Redundancy	3.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% PEG 3350, 0.2M potassium nitrate