5YXT

Crystal structure of reducing end xylose-releasing exo-oligoxylanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-29
Detector	RAYONIX MX-225
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	54.570, 78.990, 89.148
Unit cell angles	100.34, 95.86, 106.35

Refinement procedure

Resolution	22.350 - 1.880
R-factor	0.2454
Rwork	0.244
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wu4
RMSD bond length	0.007
RMSD bond angle	0.825
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2474: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.350	1.940
High resolution limit [Å]	1.880	1.880
Rmerge	0.074
Number of reflections	105939
<I/σ(I)>	5.2
Completeness [%]	93.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium thiocyanate, PEG 3350