5YXG
Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.106, 83.160, 149.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.660 - 1.480 |
| R-factor | 0.17807 |
| Rwork | 0.177 |
| R-free | 0.19534 |
| Structure solution method | SAD |
| Starting model (for MR) | 5yu5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.660 | 1.620 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.063 | 0.536 |
| Rmeas | 0.070 | 0.596 |
| Rpim | 0.029 | 0.253 |
| Number of reflections | 104530 | 24710 |
| <I/σ(I)> | 15.6 | 3.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.3 | 5.2 |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium chloride 20% w/v PEG 3350 |
