5YXF

Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105L mutant) in complex with Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-12-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	49.302, 48.326, 56.225
Unit cell angles	90.00, 95.19, 90.00

Refinement procedure

Resolution	35.370 - 1.610
R-factor	0.1714
Rwork	0.169
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yj3
RMSD bond length	0.020
RMSD bond angle	2.038
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.370	36.000	1.670
High resolution limit [Å]	1.610	3.470	1.610
Rmerge	0.055	0.060	0.350
Rmeas	0.076	0.082	0.483
Rpim	0.052	0.057	0.331
Number of reflections	33848	6685	5684
<I/σ(I)>	17.7
Completeness [%]	96.5	99.7	84.9
Redundancy	1.9	1.9	1.7
CC(1/2)	0.986	0.979	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes pH 7.5, 27% Peg3350, 3% glycerol