5YXE
Crystal Structure Analysis of feline serum albumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.908, 49.145, 123.869 |
Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
Resolution | 46.795 - 3.402 |
R-factor | 0.286492746435 |
Rwork | 0.286 |
R-free | 0.29674 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lu8 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.493 |
Data reduction software | XDS (May 1, 2016) |
Data scaling software | XDS (May 1, 2016) |
Phasing software | MOLREP (11.2.08) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 3.610 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 8375 | 2486 |
<I/σ(I)> | 3.7 | |
Completeness [%] | 97.0 | |
Redundancy | 3.4 | |
CC(1/2) | 0.973 | 0.617 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 293 | 47mg/mL FSA in 10mM Na Phosphate pH7.4, 35% PEG3350, 0.2M Na malonate pH4.8 |