5YX4
Isoliquiritigenin-complexed Chalcone isomerase (S189A) from the Antarctic vascular plant Deschampsia Antarctica (DaCHI1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2016-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97950 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.221, 83.221, 93.127 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.610 - 2.100 |
R-factor | 0.19073 |
Rwork | 0.189 |
R-free | 0.22602 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.945 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 22153 | |
<I/σ(I)> | 40.4 | |
Completeness [%] | 99.5 | |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 0.076 M sodium phosphate dibasic, 1.152 M potassium phosphate dibasic (pH 8.2) |