5YWW
Archael RuvB-like Holiday junction helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 69.116, 74.766, 107.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.330 |
| R-factor | 0.20966 |
| Rwork | 0.207 |
| R-free | 0.23642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f4h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.577 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.330 |
| Rpim | 0.046 | 0.414 |
| Number of reflections | 24438 | |
| <I/σ(I)> | 32.38 | 2.44 |
| Completeness [%] | 99.4 | 97.1 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 287 | 0.1M Bis-tris pH 6.5, 20% PEG monomethyl ether 5000 |
