5YWN
SsCR_L211H-NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.782, 53.093, 70.768 |
| Unit cell angles | 90.00, 95.50, 90.00 |
Refinement procedure
| Resolution | 35.221 - 2.039 |
| R-factor | 0.1685 |
| Rwork | 0.166 |
| R-free | 0.22300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.221 | 2.080 |
| High resolution limit [Å] | 2.039 | 2.040 |
| Number of reflections | 21629 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.3 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M lithium sulfate monohydrate, 0.1M Tris hydrochloride (pH 8.0), 33% (w/v) PEG 4000 |
