5YWI
Crystal structure of Arabidopsis thaliana HPPD Truncated mutant complexed with NTBC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.601, 83.568, 64.150 |
Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
Resolution | 29.776 - 2.580 |
R-factor | 0.2553 |
Rwork | 0.254 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tfz |
RMSD bond length | 0.009 |
RMSD bond angle | 0.713 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.059 | 0.432 |
Rmeas | 0.073 | 0.537 |
Rpim | 0.043 | 0.314 |
Number of reflections | 11361 | 504 |
<I/σ(I)> | 20.36 | 1.54 |
Completeness [%] | 90.5 | |
Redundancy | 2.6 | 2.4 |
CC(1/2) | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.16 | 0.1M Tris-HCl-Bicine pH8.5, 12.5% MPD, 12.5% PEG 1000, 12.5% PEG 3350, 0.03M NaF, 0.03M NaBr, 0.03M NaI |