5YVQ
Complex of Mu phage tail fiber and its chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 66.332, 66.332, 387.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.140 - 2.103 |
| R-factor | 0.2378 |
| Rwork | 0.236 |
| R-free | 0.26380 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.164 | |
| Rmeas | 0.174 | |
| Rpim | 0.073 | 0.624 |
| Number of reflections | 59378 | |
| <I/σ(I)> | 14.3 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9.2 |
| CC(1/2) | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | (NH4)2SO4, 2-Methyl-2,4-pentanediol |
