5YVP

Crystal structure of an apo form cyclase Filc1 from Fischerella sp. TAU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-04
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	86.758, 255.893, 34.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.242 - 2.051
R-factor	0.214
Rwork	0.212
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yvk
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.073	0.052	0.400
Rmeas	0.082	0.059	0.449
Rpim	0.038	0.028	0.201
Number of reflections	49655	5160	4856
<I/σ(I)>	15.6
Completeness [%]	99.1	96.3	99.9
Redundancy	4.9	4.9	4.9
CC(1/2)		0.994	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	1.0M sodium citrate, 0.1M Imidazole pH 8.0