5YVN
Human Glutathione Transferase Omega1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-10-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.226, 57.226, 140.051 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.732 - 1.330 |
R-factor | 0.1757 |
Rwork | 0.175 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eem |
RMSD bond length | 0.008 |
RMSD bond angle | 1.086 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.350 |
High resolution limit [Å] | 1.330 | 1.330 |
Number of reflections | 60978 | |
<I/σ(I)> | 38.88 | |
Completeness [%] | 98.4 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 288 | 2 M Ammonium sulfate, 100 mM Sodium acetate |