5YVK

Crystal structure of a cyclase Famc1 from Fischerella ambigua UTEX 1903

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-26
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	54.845, 54.845, 152.562
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.137 - 1.292
R-factor	0.1661
Rwork	0.166
R-free	0.18740
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.396
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.340
High resolution limit [Å]	1.290	2.780	1.290
Rmerge	0.055	0.037	0.532
Rmeas	0.057	0.039	0.571
Rpim	0.016	0.011	0.201
Number of reflections	67572	7140	6491
<I/σ(I)>	13.8
Completeness [%]	99.6	99.3	96.6
Redundancy	11.8	11.6	6.8
CC(1/2)		0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	1.0M sodium citrate, 0.1M Imidazole pH 8.0