5YVD
Structural and biochemical characterization of endoribonuclease Nsp15 encoded by Middle East Respiratory Syndrome Coronavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.643, 145.643, 96.653 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.590 - 2.700 |
| R-factor | 0.17875 |
| Rwork | 0.175 |
| R-free | 0.24656 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.699 |
| Number of reflections | 21016 | |
| <I/σ(I)> | 6.33 | |
| Completeness [%] | 98.7 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.952 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 4% v/v tacsimate, pH 4.0, 12% w/v polyethylene glycol 3350 |
