5YV5

Crystal structure of the complex of archaeal ribosomal stalk protein aP1 and archaeal ribosome recycling factor aABCE1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	60.011, 64.731, 147.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.100
R-factor	0.19695
Rwork	0.194
R-free	0.24376
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yqt
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BALBES
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.980	2.220
High resolution limit [Å]	2.100	2.100
Number of reflections	31682
<I/σ(I)>	21.03
Completeness [%]	97.2
Redundancy	3.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% (w/v) PEG 4000, 10% (v/v) 2-propanol, 0.1 M HEPES pH 7.0