5YV3
DNA polymerase IV - DNA ternary complex 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.249, 57.038, 109.950 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 54.802 - 2.030 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.761 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.600 | 2.240 |
| High resolution limit [Å] | 2.030 | 2.120 |
| Rmerge | 0.075 | 0.610 |
| Number of reflections | 69290 | 8251 |
| <I/σ(I)> | 12.3 | 2.4 |
| Completeness [%] | 98.3 | 93.9 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.997 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 15% MPD , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
