5YV1

DNA polymerase IV - DNA ternary complex 13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-18
Detector	MAR CCD 130 mm
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	86.070, 57.100, 110.300
Unit cell angles	90.00, 94.59, 90.00

Refinement procedure

Resolution	54.973 - 2.090
R-factor	0.1989
Rwork	0.196
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ir1
RMSD bond length	0.008
RMSD bond angle	1.041
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.160	2.200
High resolution limit [Å]	2.090	2.090
Rmerge	0.069	0.578
Number of reflections	63709	9296
<I/σ(I)>	11.9	2.6
Completeness [%]	100.0	100
Redundancy	4.4	4.4
CC(1/2)	0.998	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	15% MPD, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K