5YUS
DNA polymerase IV - DNA ternary complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-16 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.840, 57.120, 110.550 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 44.721 - 1.940 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.019 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.120 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.571 | |
| Number of reflections | 80323 | 11636 |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.4 | 4.4 |
| CC(1/2) | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 15% MPD , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
