5YUB
Crystal structure of voltage-gated sodium channel NavAb E32Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-05 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 127.040, 127.040, 202.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.062 - 3.400 |
R-factor | 0.243872770287 |
Rwork | 0.241 |
R-free | 0.27284 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yua |
RMSD bond length | 0.011 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.070 | 3.521 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.243 | 1.924 |
Rpim | 0.069 | 0.543 |
Number of reflections | 11773 | |
<I/σ(I)> | 14.86 | |
Completeness [%] | 86.4 | |
Redundancy | 13.9 | |
CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% polyethylene glycol monomethyl ether 2000, 100 mM NaCl, 100 mM calcium nitrate, 100 mM Tris-HCl, pH 8 |