5YU5
Crystal structure of shaft pilin spaD from Lactobacillus rhamnosus GG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953720 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.412, 76.264, 400.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 200.000 - 2.270 |
| R-factor | 0.20348 |
| Rwork | 0.202 |
| R-free | 0.23616 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.536 |
| High resolution limit [Å] | 2.270 | 2.273 |
| Rmerge | 0.046 | 0.473 |
| Rmeas | 0.051 | 0.533 |
| Rpim | 0.021 | 0.236 |
| Number of reflections | 39269 | 1964 |
| <I/σ(I)> | 18.1 | 2.7 |
| Completeness [%] | 87.1 | 48.5 |
| Redundancy | 5.9 | 4.5 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES pH 6.5, 30% w/v PEG 400 |
