5YU2
Structure of Ribonuclease YabJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.118, 83.216, 89.358 |
| Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
| Resolution | 46.990 - 1.750 |
| R-factor | 0.16181 |
| Rwork | 0.160 |
| R-free | 0.20206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qd9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.888 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.4.06) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 65604 | |
| <I/σ(I)> | 42.6 | |
| Completeness [%] | 99.1 | 95.1 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 25% (w/v) PEG 3350, 100 mM Tris/HCl, pH 8.5 |
