5YTX

Crystal structure of YB1 cold-shock domain in complex with UCAACU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 62
Unit cell lengths	66.512, 66.512, 33.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.801 - 1.551
R-factor	0.2041
Rwork	0.200
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pf5
RMSD bond length	0.006
RMSD bond angle	0.919
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	12349
<I/σ(I)>	35.4
Completeness [%]	98.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	30 % (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium acetate, pH 4.5, 25 % (w/v) PEG 1500