5YTP
Crystal Structure of TTHA0139 L34A from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.531, 65.531, 74.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.765 - 2.457 |
R-factor | 0.2575 |
Rwork | 0.257 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ytq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.066 | 1.292 |
Rpim | 0.020 | 0.355 |
Number of reflections | 6292 | 308 |
<I/σ(I)> | 48.3 | 2.6 |
Completeness [%] | 99.5 | 100 |
Redundancy | 12.6 | 13.9 |
CC(1/2) | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 11.4mg/mL protein, 3.2M Sodium chloride, 0.1M Sodium acetate trihydrate, 4% 2,5 Hexanediol |