5YT8
Crystal structure of H5 hemagglutinin with G228S Q226L mutants from A/chicken/Taiwan/0502/2012
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 111.170, 111.170, 313.782 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.676 - 2.765 |
R-factor | 0.2022 |
Rwork | 0.199 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.870 |
High resolution limit [Å] | 2.765 | 2.765 |
Number of reflections | 19535 | 1927 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.8 | 4.8 |
CC(1/2) | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M tris, 0.2M MgCl, 14% PEG8000 |