5YT4

Galectin-10 variant H53A soaked in glycerol for 5 minutes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 65 2 2
Unit cell lengths	48.741, 48.741, 257.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.531 - 2.000
R-factor	0.1858
Rwork	0.182
R-free	0.21670
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.531	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.078	0.307
Number of reflections	13190	949
<I/σ(I)>	13.8
Completeness [%]	99.7	99.5
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG