5YT0
Crystal structure of the complex of archaeal ribosomal stalk protein aP1 and archaeal translation initiation factor aIF5B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.720, 79.016, 101.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.700 - 1.890 |
R-factor | 0.17323 |
Rwork | 0.171 |
R-free | 0.21711 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.030 |
RMSD bond angle | 2.610 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.700 |
High resolution limit [Å] | 1.900 |
Number of reflections | 35221 |
<I/σ(I)> | 26.7 |
Completeness [%] | 98.3 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M citric acid, pH 6.5, 35%(w/v) PEG600 |