5YSI

SdeA mART-C domain EE/AA NCA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	193
Detector technology	CCD
Collection date	2017-05-05
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	37.867, 75.698, 84.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.555 - 1.546
R-factor	0.208
Rwork	0.204
R-free	0.24580
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.091
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.555	1.602
High resolution limit [Å]	1.546	1.546
Rmerge		0.542
Number of reflections	36284	1754
<I/σ(I)>	32.37
Completeness [%]	99.6	97.33
Redundancy	6.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400, Tris