5YSE
Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.014, 63.280, 87.451 |
| Unit cell angles | 89.99, 82.24, 86.08 |
Refinement procedure
| Resolution | 43.320 - 1.600 |
| R-factor | 0.15902 |
| Rwork | 0.158 |
| R-free | 0.18763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ysb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.855 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.737 |
| Rpim | 0.043 | 0.474 |
| Number of reflections | 98051 | 4453 |
| <I/σ(I)> | 20.7 | 1.5 |
| Completeness [%] | 96.0 | 87 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 0.1M MES-NaOH, pH 5.3, 42% (v/v) MPD |
