5YS8

2.8 angstrom crystal structure of Succinate-Acetate Permease from Citrobacter koseri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 42
Unit cell lengths	79.874, 79.874, 88.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.610 - 2.798
R-factor	0.2505
Rwork	0.245
R-free	0.30250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ys3
RMSD bond length	0.001
RMSD bond angle	0.353
Data reduction software	XDS (v1.0)
Data scaling software	XDS (v1.0)
Phasing software	PHENIX (v1.0)
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.200
Number of reflections	13837
<I/σ(I)>	7.7	1.3
Completeness [%]	99.8	99
Redundancy	5.7	5.75
CC(1/2)	1.000	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	298	PEG 400, CaAc2, NaCl