5YS8
2.8 angstrom crystal structure of Succinate-Acetate Permease from Citrobacter koseri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 42 |
Unit cell lengths | 79.874, 79.874, 88.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.610 - 2.798 |
R-factor | 0.2505 |
Rwork | 0.245 |
R-free | 0.30250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ys3 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.353 |
Data reduction software | XDS (v1.0) |
Data scaling software | XDS (v1.0) |
Phasing software | PHENIX (v1.0) |
Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.200 | |
Number of reflections | 13837 | |
<I/σ(I)> | 7.7 | 1.3 |
Completeness [%] | 99.8 | 99 |
Redundancy | 5.7 | 5.75 |
CC(1/2) | 1.000 | 0.380 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 298 | PEG 400, CaAc2, NaCl |