5YS5
Crystal structure of Multicopper Oxidase CueO G304K mutant with seven copper ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-29 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.568, 50.632, 66.623 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ys1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.709 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.624 |
| Number of reflections | 21184 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 99.7 | 96 |
| Redundancy | 6.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2M Sodium formate (pH 8.5), 20%(w/v) Polyethyleneglycerol 3,350 |
