5YS4
Crystal structure of retroviral protease-like domain of Ddi1 from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.790, 56.780, 91.400 |
| Unit cell angles | 76.56, 84.48, 89.99 |
Refinement procedure
| Resolution | 26.340 - 2.300 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1a |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.340 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.145 | 0.800 |
| Number of reflections | 36968 | 3613 |
| <I/σ(I)> | 4.9 | 1.8 |
| Completeness [%] | 97.6 | 96.8 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.985 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.5 | 293 | 0.1M sodium malonate, 12 % polyethylene glycol 3350 |
