5YS2

Structure of the domain IV(D_IV) of Pseudorabies virus glycoprotein B( PRV gB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 2 21 21
Unit cell lengths	91.911, 119.850, 123.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.518 - 2.698
R-factor	0.23
Rwork	0.227
R-free	0.29110
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.698	2.700
Number of reflections	38191
<I/σ(I)>	15.078
Completeness [%]	99.6
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M calcium chloride dihydrate, 0.1 M MES, pH 6.0