5YRY

Crystal structure of C-terminal redox domain of APR1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	58.200, 58.200, 86.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.160 - 2.698
R-factor	0.1863
Rwork	0.179
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.160	2.800
High resolution limit [Å]	2.698	2.700
Rmeas	0.079	0.660
Number of reflections	4467	432
<I/σ(I)>	32.4	4.7
Completeness [%]	99.6	100
Redundancy	12.9	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	283	0.1 M Tris buffer pH 7.0, 1.2 M sodium citrate, 0.2 M sodium chloride