5YRQ
Crystal structure of Rad5 and Rev1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.589, 49.280, 103.074 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 29.133 - 1.999 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24630 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.815 |
| Data reduction software | HKL-2000 (v705a) |
| Data scaling software | HKL-2000 (v705a) |
| Phasing software | PHASER (v7.0) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.184 | 0.184 |
| Number of reflections | 38367 | |
| <I/σ(I)> | 16 | 6.2 |
| Completeness [%] | 95.7 | 89.4 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 18-20% PEG 3350, 100mM potassium citrate, 100mM HEPES, 5mM DTT, pH 7.0 |
