5YRP
Crystal structure of the EAL domain of Mycobacterium smegmatis DcpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 129.053, 129.053, 165.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.780 - 2.990 |
| R-factor | 0.25832 |
| Rwork | 0.256 |
| R-free | 0.30996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rnh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.959 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.800 | 3.200 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Number of reflections | 14428 | 2235 |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 8.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 8% polyethylene glycol 6000, 0.1M MgCl2, 0.1M MES, pH 6.0 |
