5YRG
PPL3A-isomaltose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-29 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.950, 50.120, 118.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.842 - 1.500 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yrk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.145 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.020 | 0.113 |
Number of reflections | 44443 | 6432 |
<I/σ(I)> | 17.6 | 5.1 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M ammonium sulfate, 25%(w/v) PEG 3350, 0.1M Tris buffer pH 5.5 |