5YQQ
Crystal structure of a domain-swapped dimer of the second StARkin domain of Lam2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 |
Unit cell lengths | 33.862, 52.124, 53.021 |
Unit cell angles | 107.81, 90.17, 105.05 |
Refinement procedure
Resolution | 24.017 - 1.900 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yqj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.862 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.045 | 0.279 |
Number of reflections | 25976 | 1273 |
<I/σ(I)> | 32.3 | 5.5 |
Completeness [%] | 97.4 | 96.6 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris-HCl pH 8.0, 30% PEG 8000 |