5YQP
Crystal structure of the second StARkin domain of Lam4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97950 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 68.337, 89.074, 111.835 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.232 - 1.700 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yqj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.923 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.071 | 0.383 |
Number of reflections | 37918 | 1880 |
<I/σ(I)> | 51.7 | 8.2 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8.8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 0.1M Bicine-NaOH pH 9.0, 30% PEG1500, 0.1M MgCl2 |