5YQM
Crystal structure of Sirt2 in complex with selective inhibitor A29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.748, 73.952, 55.947 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 44.511 - 1.735 |
| R-factor | 0.1837 |
| Rwork | 0.183 |
| R-free | 0.21960 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.257 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.735 |
| Number of reflections | 28418 |
| <I/σ(I)> | 9 |
| Completeness [%] | 99.0 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES PH6.8, 25%-30% PEG 3350 |
