5YQI

Crystal structure of the first StARkin domain of Lam2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	33.433, 59.505, 84.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.973 - 1.600
R-factor	0.2078
Rwork	0.206
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yqj
RMSD bond length	0.006
RMSD bond angle	0.746
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.427
Number of reflections	22632	1117
<I/σ(I)>	45.5	5.3
Completeness [%]	98.6	100
Redundancy	5.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1M MES-NaOH pH 6.0, 30% PEG3350, 0.1M KNO3